New Approach for Urine Tradition Improves Pathogen Detection in UTI

By | June 20, 2017

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A brand new enhanced approach for urine tradition, often called the improved quantitative urine tradition (EQUC) protocol, detected considerably extra pathogens than customary urine tradition in ladies with signs of urinary tract an infection (UTI). These examine outcomes have been offered at American Society for Microbiology (ASM) Microbe 2017, held June 1-5 in New Orleans, Louisiana.1

The usual urine tradition was initially used to determine sufferers at elevated danger of pyelonephritis. For the previous couple of many years, its use has been expanded to detecting UTIs with a cutoff of ≥105  colony-forming items (CFU)/mL of a identified uropathogen. Whereas the usual urine tradition is the gold customary for diagnosing UTI, knowledge counsel that micro organism are current in roughly 90% of ordinary urine cultures with no bacterial progress.2

Beforehand, the EQUC protocol was proven to attain increased uropathogen detection charges than the usual urine tradition (84% vs 33%).2 Researchers, led by Travis Worth, MS, and Elizabeth Mueller, MD, from Stritch Faculty of Medication, Loyola College in Chicago, Illinois, sought to determine the optimum urine tradition protocol for diagnosing UTI, the urinary signs most strongly related to UTI, and the CFU threshold for diagnosing UTI.1


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Ladies enrolled within the examine (n=150) answered “sure” or “no” to the query, “Do you’re feeling you could have a UTI?” with 75 within the “sure” group and 75 within the “no” group. Urine samples from the contributors have been cultured utilizing customary urine tradition, expanded EQUC, and streamlined EQUC protocols.1

Expanded EQUC protocols could use increased volumes of urine (1, 10, or 100 µL) and totally different progress media than the usual protocol (urine quantity = 1 µL). The streamlined EQUC protocol was developed from probably the most optimum expanded EQUC protocol to be able to present complete detection of uropathogens. The streamlined EQUC protocol requires 100 µL of urine and incubates samples in 3 progress media (blood, CNA, and MacConkey) in 5% carbon dioxide (CO2) for 48 hours.1

Within the “sure” group, solely half of uropathogens have been detected by customary urine tradition, in contrast with 91% by streamlined EQUC.1

Whereas customary urine tradition was extremely delicate for detecting Escherichia coli (detection fee, 88%), it carried out poorly for detecting non-E coli pathogens (detection fee, 12%).1

Detectable uropathogens (≥10 CFU/mL) have been current in urine samples from 69 ladies within the “sure” group. Of those, 67% had a number of pathogens with a focus of ≥105 CFU/mL, nearly all of which have been Gram-negative. Nevertheless, utilizing decrease thresholds revealed that different microbes, comparable to Gram-positive micro organism, accounted for up 62% of pathogens. UTI signs improved in most sufferers with ≥1 pathogen current at ≥105 CFU/mL who have been handled with antibiotics, however remained unchanged or worsened in 60% of sufferers with pathogens current at <105 CFU/mL who weren’t handled.1

Members additionally reported their signs with the UTI Signs Evaluation (UTISA) questionnaire. “Urinary urgency and urinary frequency usually are not signs which are particular for UTIs,” Dr Mueller instructed Infectious Illness Advisor. “The ‘greatest’ symptom is dysuria or ache with urination.”

The EQUC protocols could detect extra pathogens than customary urine tradition, however their utility within the medical setting has not been established. Dr Mueller indicated that she and her colleagues are creating a medical trial to find out whether or not utilizing EQUC protocols to diagnose UTI in ladies who really feel they’ve a UTI results in improved signs. 

References

  1. Worth TK, Dune T, Hilt EE, et al. Detecting clinically related microorganisms: we will do higher. Offered at: ASM Microbe 2017; New Orleans, LA; June 1-5. Session 206-CPHM03. Poster 459.
  2. Worth TK, Dune T, Hilt EE, et al. The medical urine tradition: enhanced strategies enhance detection of clinically related microorganisms. J Clin Microbiol. 2016;54(5):1216-1222. doi:10.1128/JCM.00044-16

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